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Living Modified Organism
(LMO)
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Phytophthora-resistant potato
EN
SP951
No
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- Organization: Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development (ICABIOGRAD) | BCH-CON-SCBD-259139-2Organization:Indonesian Center for Agricultural Biotechnology and Genetic Resource Research and Development (ICABIOGRAD)Academic or research instituteBB Biogen, Jl. Tentara Pelajar No. 3ABogor, Jawa Barat
16111, IndonesiaPhone: +62 251.8337975,Fax: +62 251.8338820,Email: bb_biogen@pertanian.go.id,Website: http://biogen.litbang.pertanian.go.id/,
The potato (Solanum tuberosum) was modified for Phytophthora infestans resistance through the expression of Solanum bulbocastanum late blight resistance gene 1, which confers broad resistance against P. infestans races. In addition, the modified potato contains an Escherichia coli neomycin phosphotransferase II cassette for kanamycin (or neomycin) selection during transformation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Wild-type 'Katahdin'
EN
pCLD04541
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100271-5 Octopine Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103777-3 Phytophthora infestans Resistance gene 1 Promoter | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Promoter
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BCH-GENE-SCBD-102155-7 Phytophthora infestans Resistance gene 1 | Solanum venturii (Wild Potato, SOLVN)Protein coding sequence | Resistance to diseases and pests (Fungi)
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BCH-GENE-SCBD-103778-3 Phytophthora infestans Resistance gene 1 terminator | Solanum bulbocastaneum (Nightshade, Ornamental nightshade)Terminator
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
The modified potato contains two gene cassettes: Escherichia coli neomycin phosphotransferase II (nptII) and Solanum bulbocastanum Phytophthora infestans resistance gene 1 (rpi1).
The nptII coding sequence is under control of a Cauliflower mosaic virus 35S promoter and an Agrobacterium tumefaciens octopine synthase terminators. High levels of transcription are expected due to the constitutive nature of the promoter.
The rpi1 gene was inserted under its own (rpi1) promoter and terminator.
Note:
The nptII coding sequence is under control of a Cauliflower mosaic virus 35S promoter and an Agrobacterium tumefaciens octopine synthase terminators. High levels of transcription are expected due to the constitutive nature of the promoter.
The rpi1 gene was inserted under its own (rpi1) promoter and terminator.
Note:
- Southern analysis confirmed that the parental genome contains a singular T-DNA insertion.
EN
- Food
EN
EN
- EUginius - SP951 potato [ English ]
- Journal of the American Society for Horticultural Science - Late Blight Resistance of RB Transgenic Potato Lines.pdf [ English ]
- Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight.pdf [ English ]
- Penentuan Alergenisitas Protein Gen RB pada Kentang Produk Rekayasa Genetika Berdasarkan Studi Bioinformatika.pdf [ Indonesian ]
- Genetic stability analysis of RB gene in genetically modified potato lines.pdf [ English ]
- Genotypic background of the recipient plant is crucial for conferring RB gene mediated late blight resistance in potato.pdf [ English ]
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